Metagenomic Next-Generation Sequencing for severe pneumonia after allogeneic transplantation Diagnosis: Lung Biopsy versus Bronchoalveolar Lavage Fluid
J. Junhong*a (Prof), W. Ila (Ms)
a Department of Pulmonary and Critical Care Medicine, Dushu Lake Hospital Affiliated to Soochow University, Suzhou, China, Suzhou, CHINA
Purpose: To discuss the value with metagenetic second-generation sequencing (mNGS) from different sources specimens, about patients diagnosed with severe pneumonia after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Method: A total of 54 patients with severe pneumonia after allo-HSCT from April 2018 to October 2021 were included in this retrospective study. All patients’ specimens in the same site were collected through bronchoscopy. All completed mNGS detection. Compared the performance of two specimen assays for mNGS detection in diagnosis and pathogen identification, comparing differences in the types of pathogenic bacteria detected by the two samples. Results: In 54 patients who underwent bronchoscopy, none of them developed severe complications. The positive rate of mNGS was 74.1% in lung tissue and 66.7% in BALFmNGS. The positive rate of combined detection of the two was significantly increased. In bacteria, viruses and fungi, respectively, the positive detection rate of the two samples was not statistically significant. Conclusion: In patients with severe pneumonia after allo-HSCT, there was no significant difference in the diagnostic performance between lung tissue mNGS and BALF mNGS, and the positive rate of the combination of the two was high, while its negative rate could rule out the diagnosis to some extent.
Disclosure of funding source(s): none