Identification of potential genes in endobronchial tuberculosis afterbronchoscopic cryotherapy by transcriptome sequencing    

Y. Zhou*^a (Dr)

^a Xi'an Chest Hospital, Xi'An,shaanxi, CHINA

* 18729378033@163.com

Background: Bronchoscopic cryotherapy facilitates the treatment of endobronchial tuberculosis (EBTB) and helpssuppress progressive bronchial stenosis. However, the molecular mechanism of bronchoscopic cryotherapy inEBTB patients has not been reported. Methods: Transcriptome sequencing was performed to explore differentially expressed mRNAs (DEGs) in EBTBpatients before and after bronchoscopic cryotherapy. Gene Ontology (GO) and KEGG analyses were carried out.Five genes(MKLN1, HIGD1A, PTGES, SKIL, and MCEMP1) were selected and validated using real-time qPCR (RT-qPCR). Results:In transcriptome analysis, 448 DEGs with p < 0.05 and|logFC| > 1 were identified; of these,171 and 277DEGs were significantly up-and down-regulated after bronchoscopic cryotherapy, respectively. Results displayed337 biological process (GO-BP), 48 cellular component (GO-CC) and 62 molecular function (GO-MF) terms and20 KEGG pathways. RT- qPCR results were consistent with the sequencing data. Conclusion: These data provide informative evidence and recommendations for further scientific research onbronchoscopic cryotherapy for EBTB.

Disclosure of funding source(s):

This study is supported by Xi'an Municipal Bureau of Science andTechnology [20YXYJ0001(4)].