Therapeutic effect and mechanism of bronchoscopic cryoablation on airway stenosis caused by tracheal cartilage injury in rabbits
J. Junhong*a (Prof), W. Ila (Ms)
a Department of Pulmonary and Critical Care Medicine, Dushu Lake Hospital Affiliated to Soochow University, Suzhou, China, Suzhou, CHINA
* jiang20001969@163.com
Objective: To establish a rabbit model of tracheal chondrocyte injury, and to observe the therapeutic effect of cryoablation on cartilage injury-induced airway stenosis by EB-OCT analysis under bronchoscopy.
METHODS: Rabbit tracheal chondrocyte injury model was established and identified. Western blot, real-time quantitative PCR, flow cytometry TUNEL technology, and CCK8 method were used to detect chondrocyte injury at 4 time points before and 1, 3, and 5 days after injury. Collagenase type (coll-II), matrix metalloproteinase 1 (MMP-1), matrix metalloproteinase 13 (MMP-13), matrix metalloproteinase inhibitor 1 (TIMP-1) mRNA expression and change trend. The characteristics of bronchoscopy and EB-OCT images were observed in animals at 2, 4, and 6 weeks after operation.
Results: Rabbit tracheal chondrocytes were successfully cultured, and a chondrocyte injury model was established. The gray value of MMP-1 and MMP13 in the freezing group and the injured group gradually increased after modeling, and the gray value of COL-II gradually decreased. After injury, the values of MMP-1 and MMP13 in the freezing group increased and then decreased, and COL-II decreased and then increased gradually compared with the injury group, and the difference was statistically significant. After injury, the apoptosis rate of the cryoablation group was significantly lower than that of the injury group, and the difference was statistically significant.
Conclusion: Rabbit tracheal chondrocytes were successfully cultured by simplified trypsin and type II collagenase sequential digestion. Compared with normal chondrocytes, the injured tracheal chondrocytes had higher expression of MMP-1 and MMP-13, and lower expression of COL-II. Cryotherapy of injured tracheal chondrocytes has a certain therapeutic effect on injured chondrocytes by inhibiting the expression of MMP-1 and MMP-13, increasing the expression of COL-II, and reducing apoptosis. Bronchoscopic cryo-freeze-thaw therapy has a certain therapeutic effect on airway stenosis caused by tracheal cartilage.
Disclosure of funding source(s): none