A new type of lung nodule ablation therapy -In vivo experiment of Contact Laser System on Beagle model.
RM. Genga (Ms), CL. Tanga (Mrs), SY. Lia (Prof), Y. Chen*a (Prof)
a Guangzhou Medical University The first affiliated Hospital, Guangzhou, CHINA
To investigate the injury and curative effect of high-temperature ablation in animal lung tissue by contact laser transbronchial ablation.
Participants: Nine beagles
Instrument: Contact Laser System（The top of the optical fiber can contact the tissue by wavelength conversion.）
Power and time: 15-20W, 10-20s (data from in vitro experiments)
The steps of the experiment:
After sedation under general anesthesia, Beagles were intubated and supported by high-flow oxygen inhalation.
Bronchoscope(external diameter 2.0mm, operating orifice 1.8mm) localization to the periphery of the left or right lower lung.
Enter the integrated laser fiber with 0.6mm diameter, select the power and time, and perform peripheral lung ablation under C-arm real-time fluoroscopy.
CT examination showed pneumothorax immediately after ablation, which confirmed that the ablation was effective.
After resuscitation, beagles were reared. 1,7,14,28 days later, a CT examination was performed, lung tissue was taken for pathological examination, and the tissue changes were observed.
After transbronchial lung tissue ablation, Beagles developed severe pneumothorax immediately. Pleural cavity puncture and aspiration and continue to observe. CT showed that pneumothorax basically disappeared after 7 days, and completely at 14 and 28 days, and there was no large area consolidation at the ablation site. Pathological sections showed that granulation hyperplasia of lung tissue filled the ablation cavity and formed tissue repair after 7 days.
The new Contact Laser System is different from the traditional ablation methods such as RFA, microwave, APC, and argon-helium cryoablation, which can instantly reach a high temperature above 300℃ and achieve tissue combustion and gasification ablation. In vivo ablation showed the accurate range of thermal injury, tissue cavity was formed immediately after ablation, edge carbonation, long-term feeding vital signs were stable, and there was no obvious thermal after-effect. Therefore, the high-temperature ablation mode has a good prospect for transbronchial nodule ablation.
Disclosure of funding source(s): none