Efficacy and mechanism of submucosal local injection of triamcinolone acetonide in the treatment of Tracheobronchial stenosis due to tuberculosis

Y. Xiao*^a (Dr)

^a Hunan Chest Hospital, Changsha, CHINA

* xybsnow@163.com

Objective To evaluate the clinical efficacy and mechanism of submucosal local injection of triamcinolone acetonide in the treatment of tracheobronchial stenosis due to tuberculosis. Methods 1.A total of 110 eligible patients with tracheobronchial tuberculosis (TBTB, stenosis ≥50%) in our hospital from January 2019 to December 2021 were included in this study. In the observation group，58 patients were treated by conventional interventional therapy combined with local injection of TA，and 52 patients received conventional interventional therapy alone in the control group. The clinical efficacy was statistically compared between two groups. 2. Use immunohistochemistry to compare the the expression of IL-8 and bFGF in airway mucosa tissue. Human primary tracheal fibroblasts were co-incubated with different concentrations of TA , Ag85B was used to stimulate cell secretion, Western blot and real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) were used to detect IL-8,bFGF protein expression levels and mRNA relative expression levels. Results: The effective diameter after dilation in observation group was higher than in control group .The 6-month re-stenosis rate was lower than control group ,The complete effective rate of treatment was higher than control group .2. Western blot and RT-qPCR detection: The positive expression levels of IL-8 and bFGF were in the Ag85B group>the blank group, while the Ag85B+TA group<Ag85B group . CONCLUSION: Submucosal local injection of triamcinolone acetonide combined with conventional interventional therapy may reduce airway inflammation and fibrosis by down-regulating the expression of IL-8 and bFGF, thereby inhibiting the re-stenosis of TSTB after dilation , be safe and effective.

Disclosure of funding source(s):

Fund program： Clinical Medical Technology Innovation Guidance Project of Hunan Province(2020SK50701);Natural Science Foundation of Changsha City(kq2014187)